For the positive control in my project I need a promoter that initiated by SCF-1/CSF-1R activation. Does anyone have in mind such an optimal promoter?
Thank you.
I want to separate polysomic and non-polysomic proteins by sucrose fractionation, and then compare the fractions. If I precipitate the non-polysomic fraction by TCA standards directly from the...
09 October 2013 4,952 7 View
I’m looking for site or software that maps all known micro-RNAs coding sequences. I would like to know if the genomic region that I have got from GWAS coding or any interesting micro-RNA.
03 April 2013 7,583 3 View
I have a problem with luciferase-expression assay. It is very simple: I take the promoter of interest, insert it before luciferase coding sequence, and get great results! When I over-express the...
31 December 2012 3,233 29 View
My question is about PCR. Simple, nothing special, genotyping PCR. When I’m performing the reaction on my bench, the NTC appears contaminated. On the bench in another lab, with the same pippetors...
31 December 2012 9,900 9 View
I want to knock down CSF-1 (M-CSF) in H358 human lung cancer cells. I bought shRNA from Sigma, a validated one, and checked that it has a seeding sequence in all 4 splicing variants of the CSF...
31 December 2012 2,611 6 View
How can I deplete endogenous microRNA in cell line?
06 July 2012 460 6 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View