7 Questions 17 Answers 0 Followers
Questions related from Lena Ilan
I want to separate polysomic and non-polysomic proteins by sucrose fractionation, and then compare the fractions. If I precipitate the non-polysomic fraction by TCA standards directly from the...
10 October 2013 4,955 7 View
I’m looking for site or software that maps all known micro-RNAs coding sequences. I would like to know if the genomic region that I have got from GWAS coding or any interesting micro-RNA.
04 April 2013 7,589 3 View
I have a problem with luciferase-expression assay. It is very simple: I take the promoter of interest, insert it before luciferase coding sequence, and get great results! When I over-express the...
01 January 2013 3,241 29 View
My question is about PCR. Simple, nothing special, genotyping PCR. When I’m performing the reaction on my bench, the NTC appears contaminated. On the bench in another lab, with the same pippetors...
01 January 2013 9,906 9 View
I want to knock down CSF-1 (M-CSF) in H358 human lung cancer cells. I bought shRNA from Sigma, a validated one, and checked that it has a seeding sequence in all 4 splicing variants of the CSF...
01 January 2013 2,628 6 View
How can I deplete endogenous microRNA in cell line?
07 July 2012 467 6 View
For the positive control in my project I need a promoter that initiated by SCF-1/CSF-1R activation. Does anyone have in mind such an optimal promoter? Thank you.
05 May 2012 1,494 0 View
