For bacteria, I always used 20% Glycerol to freeze them at -70 to -80°C. Worked like a charm for competent cells.

Nice work Martin,

Please read this articles, I think it will be helpful for you, even 10% :)

Also, I m thinking that Sebastian has right about 20% Glycerol use......best

Vesna

For an excellent review on cryoprotectants : see http://zoologia.biologia.uasnet.mx/protozoos/protozoa41.pdf

For an excellent review on cryoprotectants : see http://zoologia.biologia.uasnet.mx/protozoos/protozoa41.pdf

Dear Martin,

Here is some useful info for cryoprotectant:

http://www.ncbi.nlm.nih.gov/pubmed/15177901