Martin Williams

7 Questions 13 Answers 0 Followers

Questions related from Martin Williams

How to count red blood cells in countess II FL?

Hello, I am trying to count Red Blood Cells in Invitrogen's Countess II FL equipment. Which parameters do I need to set up? Many thanks!

05 May 2023 5,268 0 View

Mammalian Cell Electroporation using BTX EXM 830?

Hello! I am trying to use BTX ECM830 electroporator to introduce plasmid DNA into mammalian cells. I have tried using DMEM, HEPES-Based buffer and PBS, without success. i have used between 50 and...

10 April 2023 7,475 5 View

PEI DNA in-vivo transfection concentration?

I have bought 1 gram powder 40 kDa (22 kDa free base) PEI from Polysciences and I´m planning to use it for in vivo DNA transfection in a veterinary clinical trial. How is PEI mM calculated in...

05 January 2021 7,102 7 View

Mammalian cell culture electroporation?

Hello, does anybody has any electroporation protocol for transfecting the tumoral cell lines MDA-MB 231 and SK-MEL 28 or others? Many thanks!

09 July 2017 8,554 1 View

Eukaryote Promoter consensus sequences analysis ?

Is there any easy-to-use software to analyze core promoter regions, (TATA, INR, DPE) and other distal promoter regions or enhancers ? Any paper or review of eukaryotes promoters? Many thanks! Martin

08 November 2016 3,383 2 View

Tumoral cell line intradermical nude mice inoculation?

Hello, I need to generate tumors in nude mice with the cell lines SK MEL 28 (melanoma) and MDA MB 231 (breast cancer), both human tumoral cell lines. How many cells do i have to inject...

10 June 2016 9,663 3 View

Store competent E coli at -20 ºC?

Is there any posibility to store chemical competent or electrocompetent E coli al -20 ºC? Any protocol? Thanks

09 December 2015 5,689 7 View