6 Questions 11 Answers 0 Followers
Questions related from Martin Williams
Hello, I am trying to count Red Blood Cells in Invitrogen's Countess II FL equipment. Which parameters do I need to set up? Many thanks!
05 May 2023 5,260 0 View
Hello! I am trying to use BTX ECM830 electroporator to introduce plasmid DNA into mammalian cells. I have tried using DMEM, HEPES-Based buffer and PBS, without success. i have used between 50 and...
10 April 2023 7,466 5 View
I have bought 1 gram powder 40 kDa (22 kDa free base) PEI from Polysciences and I´m planning to use it for in vivo DNA transfection in a veterinary clinical trial. How is PEI mM calculated in...
05 January 2021 7,074 7 View
Hello, does anybody has any electroporation protocol for transfecting the tumoral cell lines MDA-MB 231 and SK-MEL 28 or others? Many thanks!
09 July 2017 8,550 1 View
Is there any easy-to-use software to analyze core promoter regions, (TATA, INR, DPE) and other distal promoter regions or enhancers ? Any paper or review of eukaryotes promoters? Many thanks! Martin
08 November 2016 3,377 2 View
Hello, I need to generate tumors in nude mice with the cell lines SK MEL 28 (melanoma) and MDA MB 231 (breast cancer), both human tumoral cell lines. How many cells do i have to inject...
10 June 2016 9,647 3 View
