12 Questions 15 Answers 0 Followers
Questions related from Martin Williams
Hello, I am trying to count Red Blood Cells in Invitrogen's Countess II FL equipment. Which parameters do I need to set up? Many thanks!
05 May 2023 5,309 0 View
Hello! I am trying to use BTX ECM830 electroporator to introduce plasmid DNA into mammalian cells. I have tried using DMEM, HEPES-Based buffer and PBS, without success. i have used between 50 and...
10 April 2023 7,519 5 View
Hello, is there any method or buffer for mammalian cell line detachment from flask for subculture? Any in-house prepared solution? Thanks!
25 February 2022 5,373 1 View
I have bought 1 gram powder 40 kDa (22 kDa free base) PEI from Polysciences and I´m planning to use it for in vivo DNA transfection in a veterinary clinical trial. How is PEI mM calculated in...
05 January 2021 7,216 7 View
Is there a method for estimating the OD of an E. coli culture without using the spectrophotometer? [I need to estimate an OD600 = 0,4 - 0,8 for electrocompetent e coli]
14 August 2020 5,273 4 View
Hello, does anybody has any electroporation protocol for transfecting the tumoral cell lines MDA-MB 231 and SK-MEL 28 or others? Many thanks!
09 July 2017 8,596 1 View
Is there any easy-to-use software to analyze core promoter regions, (TATA, INR, DPE) and other distal promoter regions or enhancers ? Any paper or review of eukaryotes promoters? Many thanks! Martin
08 November 2016 3,430 2 View
Hello, I need to generate tumors in nude mice with the cell lines SK MEL 28 (melanoma) and MDA MB 231 (breast cancer), both human tumoral cell lines. How many cells do i have to inject...
10 June 2016 9,741 3 View
Is there any posibility to store chemical competent or electrocompetent E coli al -20 ºC? Any protocol? Thanks
09 December 2015 5,728 7 View
Hello, i´m trying to dry DNA, is there any buffer to dry DNA and then resuspend it with no problem? Thanks
03 December 2015 8,500 4 View
I need to make Master E coli cultures but i cant keep them at -80. Is there any way to lyophilize transformed E coli? Thanks
10 November 2015 4,887 1 View
Hello, i want to know wich cryoprotectant is the best and in with % to freeze: Bacteria at -70 Eucariotic cells in liquid nitrogen And besides that, Is there any protocol to freeze bacteria at -20...
19 April 2015 7,107 5 View
