How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

How to measure CPT1/FAO activity in a rare cell type?

Hello all, I am searching for a way to measure the activity of CPT1 and/or FAO in mouse hematopoietic stem cells, which means I have very little material available to do so. Any...

20 January 2021 8,499 2 View

How can I verify the design on the FPGA ZedBoard?

I have designed AXI Interconnect to connect 4 masters and 1 slave using SystemVerilog and Vivado Tool. The simulation stage of the design is working well. In the design, there are more I/O pins...

06 January 2021 8,487 2 View

Transfecting high molecular weight poly I:C using lipofectamine 2000?

I'm planning to transfect HepG2 and Huh7 with poly I:C (high molecular weight poly IC from Invivogen using lipofectamine 2000 as transfectant reagent. I would like to do a standard curve using...

01 January 2021 239 1 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View

How to determine the dominant orientation of collagen fibers.?

what is the best method to determine the dominant orientation of collagen fibers? i preformed FFT and shited the frequencies , what should be my next step ?

24 December 2020 3,360 3 View

Is it possible to calculate the sample size of a clinical trial with the Cohen formula when there is no similar article?

We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...

21 December 2020 2,454 3 View

Has anybody had staining problems with lysotracker in live cells?

I am working with two fish cells types, a zebrafish liver cell line and primary cultures of trout macrophages. We are using lysotracker to stain them in order to visualize lysosomes in confocal...

16 December 2020 3,935 2 View

Is anyone share your experience in making hydro gel using bovine collagen?

dear all, Actually, I m trying to prepare hydrogel using fish collagen, rat tail collagen, and bovine collagen to check the cell adhesion and viability. fish and rat tail collagen nicely formed as...

15 December 2020 7,142 4 View