I am doing the second step electroporation of the CRISPR/Cas9 system, which is inserting my target linear fragment with homology arms and the custom-designed gRNA plasmid into a bacteria that has both Cas9 and the vector which needs to be cut.

I grow the electroplated cells on a medium that has the antibiotic for the vector, Cas9, and the gRNA plasmid but unfortunately cannot get colonies. Can you please help what I can improve in this experiment?

Thanks in advance