Melika Irani

2 Questions 3 Answers 0 Followers

Questions related from Melika Irani

CRISPR/Cas9 second repairing electroporation troubleshooting?

I am doing the second step electroporation of the CRISPR/Cas9 system, which is inserting my target linear fragment with homology arms and the custom-designed gRNA plasmid into a bacteria that has...

18 March 2021 3,481 1 View

I do the restriction digest as verification for my plasmid. Why sometimes can't I get bands, even for the uncut sample?

I measured the DNA concentration using Nanodrop and made 10 μl total digest solution. I was wondering what would be the problem that I don't even get band for the uncut control sample?!

04 February 2021 742 6 View