Our LAB is BSL-1 level, can we use SARS-CoV-2 Pseudovirus in BSL-1 lab?
No, for working on SARS-CoV-2 pseudovirus you need BSL-3. BSL-1 can be used only for non-contagious microbes.
SARS-CoV-2 pseudovirus is a virus that lacks virulent genes, so it is safer than the intact one, but it needs biosafety level 2 not one
IMO, Mohammad's answer is correct, following an appropriate risk assessment signed off by whatever local authority governs the lab.
If the pseudovirus is replication defective outside of the producer cell line (which it must be, by definition) and has been designed in such a way that recombination to produce an intact SARS-CoV-2 genome is impossible (which it should be) then there's no reason to handle at CL3. Most Institutions would want it to be handled at CL2 however, because it's still an infectious virus (albeit single round) and you don't want your staff exposed to such a thing for all sorts of reasons.
Pseudo viruses do not replicate and they lack virulent genes that's why in my opinion they are not dangerous like real virus but to study them one should must use BSL-2 . As BSL-1 is used for non pathogenic bacterial strains and public access is also not limited in it.
