I've seen a lot of different protocols for coupling antibodies into gold nanoparticles, but mostly for polyclonal antibodies. Does anyone know an efficient protocol for conjugation of colloidal gold into monoclonal IgG and IgM, obtained from mouse? We would like to perform an IFLA test, but most of the problem is in the ammount necessary to conjugate and which buffer to use. Can someone, plz, help me with that?