Hello everyone,
I want to know how many cells are in my glycerol stocks. They were all made in the same way, and they yield similar results, but I kind of forgot about one step or two before making them, which means that I don't know how many microorganisms there are "originally".
Would that be okay to simply unthaw couple of stocks, conduct a serial dilution (100 ul from a stock into 900 ul of saline solution) and just follow the steps similarly to SP-SDS or 6x6 method? Is there necessity of "bacterial activation", given that they will be incubated on agar plate anyways?
If there is a need of "bacterial activation" - What kind of method would be the best suitable, given that I don't want to use enormous numbers of plastic plates, agar powder and wanting to just be more less-waste?
Thanking for your answers in advance,
Matty
Given that you are meant to streak out glycerol stocks onto an agar plate using a pipette tip and then pick a single colony, having the exact numbers should not be too important, as long as the stock is viable. Activation is only needed for some spore forming species.
Article How to optimize the drop plate method for enumerating bacteria
Phil Geis This is actually a good question - My stocks function as microbial strain reservoir, but recently I was just wondering "how big" this reservoir is. Other than that, I'm using them to prepare growth curves after overnight culturing as well.
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