What is the best technique to identify native bacteria groups present in soil?

I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify...

29 September 2020 4,609 9 View

Do you have any suggestion about constrained estimation of mixed models using lme4 package of R?

I would like to use the lmer function of the lme4 package to estimate the random part of the model given the coefficients of the fixed part (which have been estimated in a previous stage using...

24 February 2020 1,731 5 View

Can I use Nanostring microRNA count date (normalized or raw data) to measure relative gene expression?

Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?

25 November 2019 277 2 View

ERT Optimisation protocol sequence software-code for 3D unconventional electrode arrays?

Anyone know about any freely available software or code that can optimise protocol sequences for 3D unconventional electrodes setups, including borehole electrodes? The closest research I found...

15 November 2019 7,802 3 View

How can I calculate the concentration of 30nM miRNA from a 5nMol stock?

I have a transfection experiment to be done. The stock miRNA is 5nMol. The manufacturer recommends adding 75uL of sterile water, giving me a concentration of 66.67 uM. How can I have a final...

05 May 2019 4,141 7 View

Do we have cell culture contamination?

We are experienced lately some problems with primary cells. We could see (even in 10x magnification) tiny bright particles around the addhered cells. There is no macroscopic sign of...

14 December 2016 7,464 11 View

Could anyone help me with PCR to Mycoplasma detection?

I'm tryint to standardize a PCR to mycoplasma detection of dna from cell culture. I'm testing samples positive for mycoplasma and negative as controls. I found a weak band unspecific slightly...

29 June 2016 7,818 3 View

Low caspase activity in heavy metal lethal concentration 50 (LC50). What does that mean?

02 June 2016 1,480 3 View

Can anyone help me to calculate the NP ratio considering that I have mass per phosphate of 13,300 g/mol and mass per amino of 43 gm/mol?

Can anyone help me to calculate the NP ratio considering that I have mass per phosphate of 13,300 g/mol and mass per amino of 43 gm/mol?

22 May 2016 9,403 1 View

How do I read the results of an ANOVA (2 independent factors) that instead of F reason, I got a Z? What is this Z?

I ran a 2 independent factors ANOVA in SPSS and got a Z score instead of F. Is that related to different sample sizes in different conditions? Do I need to even the sizes of the samples before...

08 March 2016 3,813 7 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View