Could you please suggest a suitable kit for miRNA to cDNA conversion?

More Sreedevi M J's questions See All

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...

30 July 2024 3,849 0 View

I have calcined my catalysts without flowing air from the compressed air cylinder will there be any problem with my reaction if i use that catalysts?

FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion

25 July 2024 2,486 2 View

How can I measure arsenic concentration in a patient blood?

I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.

23 July 2024 3,654 2 View

Dear researchers. pl help how to plot jablonski energy level graph and magnetic hysteresis curve in origin?

through origin software

17 July 2024 4,991 0 View

It is possible to conduct a structured written interview for a qualitative study?

In your opinion, I would like to know if a qualitative study with a structured interview can be conducted in writing. In other words, we sent out open questions and asked the interviewees to...

11 July 2024 4,163 4 View

What is Differential-Fed in microstrip patch ant? How it is design in CST studio? What is process and procedure to do Differential-Fed in CST studio?

What is Differential-Fed in microstrip patch antenna ? How it is design in CST studio? What is simulation process and procedure to do Differential-Fed in CST studio ?

08 July 2024 7,464 1 View

How to know the TM/TE modes in antenna design in CST studio?

How to know the TM/TE modes in antenna design in CST studio? Example: TM01 ,TM02 ,TM10, TM12, TM30 like this... how can identify the antenna is operate the above said mode? can any one explain...

08 July 2024 6,609 2 View

What is co-polarization and cross polarization? How can plot it from CST studio?

what is co-polarization and cross polarization? How can plot it from CST studio? what are steps and process for find out co-polarization and cross polarization from CST studio?

08 July 2024 7,869 1 View

Why in some degradation experiment the results doesn't fit first or second order kinetics ?

In an antibiotic degradation experiment using metals carbon composites to activate PMS, the results doesn't fit pseudo first or second order kinetics however the degradation efficiency is very...

26 June 2024 6,941 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

In WECS : Gain values for conversion of PMSM rotor speed(rad/s) to p.u. input to wind turbine and Torque(pu) of WT to input of PMSM-Torque (Nm) ?

I am working on developing a MATLAB Simulink model of Wind turbine with PMSG followed by MPPT Boost converter. I have gone through so many literature and videos but I am not finding clear solution...

03 August 2024 888 1 View

How can one determine the conversion factor for a radiolabeled substrate to a radiolabeled product using slopes from linear regression equation?

I am preparing a radiolabeled product using a radiolabeled substrate. I have the cpm values for different dilutions of both my known substrate and unknown product ? How can one determine the...

27 July 2024 3,604 1 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

How calculate the conversion alph from tga analysis?

I read it Convert the y-axis from mass loss to degree of conversion (100-x)%. What if tga analysis have residual char, for example at mass loss% 27% Is it the same eqation to get conversion...

15 July 2024 4,218 1 View

How to convert a potential collected with reference to Saturated calomel electrode to Reversible Hydrogen Electrode?

I have collect a LSV data by taking SCE as the reference electrode. But I want to convert this one to Potential with respect to Reversible Hydrogen Electrode. Please suggest me the required...

13 July 2024 7,841 2 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

Hi all, what is the interpretation of the results of this gel electrophoresis image?

We aim to measure the RNA expression of p53 and K-RAS biomarkers in colon cancer samples. We extracted the RNA from Rneasy FFPE kit and converted the it to cDNA and did real-time PCR using Cyber...

08 July 2024 7,781 6 View

How to properly dilute RNA for cDNA?

Hi all, I have a question regarding proper dilutions and conversions of my RNA. According to my protocol, my cDNA kit is optimized for 1ug of RNA and calls for this amount. I would like to...

07 July 2024 9,206 4 View

How to change reference list (muilitple pages) from APA to Harvard?

Hello, I am looking for a tool that will do a bulk conversion of references from APA to Harvard. There are over 20 pages of references, so I would like to avoid doing them one at a time. Thank...

07 July 2024 2,003 2 View

Saurabh Mandal

Hi Sreedevi M J

My personal recommendation would be Qiagen. Qiagen provides the best product for miRNA research.

Well, based on your understanding, decide on the product.

Regards

Saurabh

Sreedevi M J

Thank you sir Saurabh Mandal

Katie A S Burnette

I also love the QIAGEN brand kits. Make sure to read the instructions carefully since there are several different ways to complete the protocol depending on the starting materials.

Thank you maam@Katie A S Burnette