We use different methods for measuring oxidative stress. But, I need a novel method, that has started being used recently. Any suggestions?
http://biyokimyadoktoru.com
The methods of determination described in the literature represent very different levels of analytical practicability, costs, and quality.
1) Measuring Free Radicals in Vivo
2)Analytical technique that directly measures free radicals is electron spin resonance (ESR) spectrometry.
3)The oxidative stress status in biological systems is based on the X-band EPR (electron paramagnetic resonance) detection of a persistent nitroxide generated under physiological or pseudophysiological conditions by oxidation of a highly
lipophylic hydroxylamine probe.
4) The quantitative measurement of the dynamic multiparametric metabolic response of living systems to pathophysiological stimuli or genetic modification.
5) Infrared laser spectroscopy is a promising method for free radical research, enabling online measurement of oxidative stress biomarkers, such as lipid peroxidation products, with high sensitivity and efficiency.
6) Lipid peroxidation is a complex process whereby polyunsaturated fatty acids (PUFAs) in the phospholipids of cellular membranes undergo reaction with oxygen to yield lipid hydroperoxides (LOOH).
7) The measurement of putative “elevated end products of lipid peroxidation” in human samples is probably the evidence most frequently quoted in support of the involvement of free radical reactions in tissue damage by disease or toxins.
8) Measurement of Thiobarbituric Acid Reacting Substances (TBARS) and Malondialdehyde (MDA)
9) Measurement of Conjugated Dienes
The methods of determination described in the literature represent very different levels of analytical practicability, costs, and quality.
1) Measuring Free Radicals in Vivo
2)Analytical technique that directly measures free radicals is electron spin resonance (ESR) spectrometry.
3)The oxidative stress status in biological systems is based on the X-band EPR (electron paramagnetic resonance) detection of a persistent nitroxide generated under physiological or pseudophysiological conditions by oxidation of a highly
lipophylic hydroxylamine probe.
4) The quantitative measurement of the dynamic multiparametric metabolic response of living systems to pathophysiological stimuli or genetic modification.
5) Infrared laser spectroscopy is a promising method for free radical research, enabling online measurement of oxidative stress biomarkers, such as lipid peroxidation products, with high sensitivity and efficiency.
6) Lipid peroxidation is a complex process whereby polyunsaturated fatty acids (PUFAs) in the phospholipids of cellular membranes undergo reaction with oxygen to yield lipid hydroperoxides (LOOH).
7) The measurement of putative “elevated end products of lipid peroxidation” in human samples is probably the evidence most frequently quoted in support of the involvement of free radical reactions in tissue damage by disease or toxins.
8) Measurement of Thiobarbituric Acid Reacting Substances (TBARS) and Malondialdehyde (MDA)
9) Measurement of Conjugated Dienes
chemiluminiscence using the “PHOTOCHEM” dedicated device produced by Jena Analytic, Germany.
by using many electrochemical method like cyclic voltametry and electron spin resonance spectroscopy we can do that. But its a hectic process, as it requires standard pure proteins also...
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