Dear All,
Can we detect a non-fluorescent organic compound (with some aromatic rings) by TLC under UV light?
For example, a compound has absorption peak at 450nm but fluorescence intensity at longer wavelength is insignificant, so can we monitor it under UV lamp?
Thank you.
www.chromatography-online.org/Detection-and-Quantitatitation/Fluorescence.php
Article Review of the first principles calculations and the design o...
For UV TLC plates the compound fluorescence is not a requirement because the UV plate is fluorescent and the absorption of the UV light shadows the plate fluorescence. If your compound has UV absorption in the range of 250-370 nm you can see black spots under the UV lamp. Because your compound contains aromatic groups you have good chances to see it. Please, also note that there are two commercially available UV active TLC sheets: 254 nm and 366 nm. My experience is that the 254 is better. Please, also note that the quantitation on the TLC by UV is not always obvious (because of the spot-spreading) unlike the charring or other visualization methods.
Like the answer given above, the molecule need not have fluorescence if you use a UV backed TLC plates. As you have mentioned that your molecule has some aromatic rings it will be definitely UV active . Use a 254nm TLC plates.
Yes, thank you all. I got misunderstanding about correlation between TLC monitor and fluorescence.
Yes, we can monitor a non-fluorescent organic compound by TLC under UV light with violet spots visible exposure to UV tube kept in a dark box.
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