How can I solve my False-Positive issues with the SARS-CoV-2 RPA amplification ?

Here, I want to ask about my issue with the SARS-CoV-2 RPA amplification False-Positive results. I tried two different sets of primers. The first sets of primers are 29 nt producing 200 bp...

20 December 2020 2,091 3 View

How can I check the correlation between a group of features and one feature?

Hello all, I have a dataset that contains some pressure meters in a water distribution network and all valves of the network. Since I do not have the graph of the water distribution network, I...

30 October 2020 2,333 4 View

Hi everyone, Can anyone kindly help me for analyzing the attached FTIR spectra?

It's an organic material used as a dispersant for refractory concretes. I had some search and found that it can be an acrylamide based compound but I am not sure yet. The datasheet supplied by the...

04 October 2020 994 9 View

Why am I getting multiple bands in western blot (in Isolating adhesion complexes) ?

Hello everyone, I am isolating adhesion complexes and wanted to confirm the presence of a specific protein in the isolated complex. When I performed western bott, I got a band at the right size...

19 September 2020 538 7 View

How can we optimize a water distribution system if we do not have the zones' topology (pipes graph )?

Hello everyone, My issue is about a water distribution system that I am working on a zone of the system where does not exist any plan for the place of pipes. However, we have the place of...

14 September 2020 5,978 6 View

Density = d = volume unit - volume of mass?

Density = d = volume unit - volume of mass? To study the vacuum status with different heavy densities reactions? Before the vacuum becomes overused? Specially in dense reactions types i.g....

28 January 2020 5,395 2 View

Oceanic controlled continental fragmentation?

Oceanic Waters center of gravitation should be attracted to the center of gravity of the Earth. However, because the water of the oceans is different from the solid mass of the Earth, the water of...

16 January 2020 177 9 View

What's the best protocol for diluting DMSO when thawing the primary human epithelial cells?

Hi, I have some problem with the viability of my primary epithelial cells after thawing. I have used two protocols (centrifuging or only diluting 10 times more and changing the media the day...

03 October 2019 7,990 6 View

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25 March 2019 4,032 3 View

What is the relation between AIDS and non-AIDS diseases among HIV positive patients?

Among HIV positive patients, is it possible that non-AIDS diseases alter the risk of developing AIDS and vice versa? For a HIV positive patient who has experienced a non-AIDS disease, Does the...

24 January 2019 1,044 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View