Could someone shed the light on differences between bond bending and bond stretches in IR specs from the FTIR analysis?

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FGT poverty index derivation?

Can anyone show me how the squared poverty gap index can be rewritten as H/n*[u2+(1-u2)CV2)] as in the picture. Step by step please. I have been struggling trying to figure out how to decompose...

07 September 2020 6,867 8 View

Can we run an EFA before a SEM?

I'm conducting a study for my PhD thesis. I've got some constructs from the literature, but the items to measure the constructs were modified (some were added and some were removed). In this case,...

08 June 2020 5,917 9 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

11 September 2019 9,441 2 View

How does freezing antibiotics result in loss of activity?

I remember my mentor saying before that she decided to refrigerate her gentamycin stock solution instead of freezing it since that was the recommended storage condition. Now, I'm dealing with...

04 August 2019 5,766 4 View

Using 5-FC for plasmid curing - confirmation steps?

To cure my Streptomyces strain of a plasmid, I used 5-FC as a negative selection marker. So, cells without the plasmid (which are the ones I'm looking for) will be able to grow on agar plates...

17 July 2019 5,854 2 View

CRISPR base-editing in bacteria not working?

I am currently using the CRISPR base editing system in bacteria. Unfortunately, I'm not getting any clones with edited bases (not even off-target) but i'm not sure why. I'm quite sure the plasmid...

29 May 2019 8,845 5 View

GDNA stored at -20?

I've extracted my gDNA from Streptomyces today, on a Friday but it's pretty late and I can't stay late today. Is it okay for me to keep my extracted gDNA over the weekends at -20 degrees Celsius...

11 April 2019 3,139 9 View

Volume of ligated product for transformation?

I've just done ligation for my plasmid and my next step would be to perform heatshock transformation on Top10 E. coli cells. Some of my colleagues recommended me to use 2 uL to transform my cells...

01 January 2019 10,003 3 View

Water or kit elution buffer to elute DNA for gel extraction?

I have PCR-ed some DNA samples which I need to perform gel extraction on. These DNA samples will subsequently be used for restriction digestion and ligation over the next week. I was advised to...

18 December 2018 4,067 7 View

Molecular cloning problems?

I've been trying to clone a ~100 bp insert into a ~12000 bp vector backbone. Basically, what I've done is to do the standard things (restriction digestion, PCR cleanup/gel extraction, ligation,...

17 December 2018 959 12 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

02 March 2021 1,653 3 View