I'm working with an animal modell based on the GVH-reaction on the hybrid system CBA(Donor)--->(receptor)[Hybrid:CBAxC57Bl],the GVH reaction is based on the H2k versus H2d genetic differences between CBA and C57Bl. I need to establish the basal level of lymphokine expression,measured by an RT-PCR test, of these mouse lines, and whant to use the minimal number of animals.Could be of great help if some body would have some accumulated reference values!!!???

Sincerely yours!

Gustavo.