In western blot, I observed the protein decrease in total AKT, PI3K 110b, PI3K classIII and some others, while actin and COX IV remains almost no change. Could this be via proteasome mechanism for multi-proteins degradation? What is the best strategy to check?
You may check it by MG132 treatment along with your drug. MG132 is a proteasomal inhibitor, if the decrease in the protein levels is through proteasome mediated degradation by your drug; MG132 will prevent it.
Moumita Sarkar, thanks for your timely response. MG132 treatment will be done. Do you familiar with the literature in which multiple proteins were degraded via ubiquitin-proteasome pathway in one treatment? I believe they could.
I do not have much experience in this domain but I consider it to be quite possible. This article in Cancer Res journal might be of help to you. Find it here
http://cancerres.aacrjournals.org/content/69/23/8967.short
Good luck
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