Hi Nancy,

An example image would be helpful. ImageJ can do a lot, but you need decent images with an analyzable contrast. The forum here may also not be the best place for this question. ImageJ has a dedicated forum (http://forum.imagej.net/) and the people there are very knowledgeable and helpful. However, they will need some example images as well.

best wishes,

Rob

if it's  individual area you can do it manually in Image J -just overcirle it 

 the same is tru for several images , not for numerous

Thank you very much Rob.

I already joined to the forum and hope they can help me with the analysis.

Nancy Marin did you manage to get an answer?