Whole mount in situ hybridzation of xnpouse embryos are destroyed/damaged while washing after the additions of probe, any idea how to prevent it?

After adding the probe/HB buffer and incubating at 65C ON there are washing steps. my embryos are getting damaged and ruptured around washing solutions 2 and 3 sometimes earlier. it doesn't help...

01 January 2020 8,993 0 View