Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

30 September 2020 2,517 1 View

Does anyone have experience with the Menstrual Attitude Questionnaire?

I am considering of using the original one from Brooke-Gunn and Ruble (1980), but maybe there is something better? I am trying to analyze the attitude of athletes towards menstruation and its...

27 August 2020 6,541 2 View

The compensation procedure- beads (for fluorochrome) and cells (dead/live staining)- how to do it?

Hi, I would like to ask about the compensation procedure. I use Fortessa/ Diva and automated compensation procedure. I have a set from BD for compensation setting. Referring to the manufacturer...

28 February 2020 8,765 2 View

What standardised psychometric test to use for self-efficacy for female athletes?

Hello, I am looking for a valid and standardised test to measure self-efficacy of female athletes for my doctoral thesis. Any suggestions? Thank you

14 November 2019 849 3 View

What is the best recommended growth and freeze media for SH-SY5Y cells?

I received SH-SY5Y WT cells and started growing them in low glucose DMEM with FBS and S/P. After reading article about changed cell morphology and detachment of cells from growth surface due to...

06 October 2019 1,475 2 View

Why can't I load my purified pcr sample from a gel to another gel to confirm purification The product evaporates out of well before I can run the gel?

23 July 2018 3,700 8 View

Why all phages in ELISA are positive?

I perform 5 biopannings rounds and then did ELISA with the 'soup of phages' from each circle (coating with specfic antybody, then phages and antiM13). Biopannings from 1-4 had really low OD but...

18 June 2018 915 3 View

Can someone help me determine the NMR data for 2,5-dimethoxyampehtamine?

Interpretation of this compound and all the chemical shifts

20 March 2017 703 1 View

Does anybody has an experience with human serum depletion prior to MS- or electrochemical analysis?

12 March 2015 8,710 5 View

Does anyone use a thermo-desorption technique for mercury speciation in solid samples?

22 February 2015 9,352 11 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View