I hoped that a murine glioma model that survives for at least 21 days is established using the GL261 cells at C57BL/6 mouse.
So, I found some papers about how to establishment using GL261 cell line.
The following are divided:
Female, C57BL/6, 5 weeks
2x10^5 cells/brain
1x10^5 cells/brain
5x10^4 cells/brain
1x10^4 cells/brain
But, 1 week after inoculation, only 60% of the tumorigenesis, and it grew so big that it died slowly and 40% didn't produce cancer.
So I used "6-week female C57BL/6 mice" to proceed again under the same group condition.
But, that survived for at 8 to 14 days.
So, I'm trying again using 8-week female C57BL/6 mice.
However, I don't know how to group and conduct the experiment.
If you've had this kind of experience, can you give me your thoughts, answers, or input?
I look forward to your answer.
Thanks.
I frequently perform intracranial injections with GL261 cells using 6 week old female C57BL/6 mice. After some optimizations of the cell number, i now inject 25000 cells in a volume of 2µl into the striatum; 2mm left of the sagittal suture and 0,5 mm anterior to the bregma at a depth of 3 mm from the dura, using a 2.5 µl Hamilton syringe equipped with an unbeveled 33G needle. With this setup i reach a 100% tumor uptake rate and an average of 26d before the onset of neurological tumor symptoms. I usually split the cells 2 days before the tumor implantation and seed them in a way that they, in the ideal case, exhibit a confluency of around 70% on the day of the implantation.Hope that information helps.
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