Dear Hero,

Thanks for your reply. The point is there is no band at all. I did change voltage but still same nothing.

Hi Hero,

The percentage I'm using is .08% and the buffer is TBE. The point is all lab member using this gel and same buffer.

Hi Hero,

The amount of DNA that I got was around 3,000ng (260:280) was around 1.92.

It is strange, given the nanodrop read was so high. Did you see smears in your gel? If so, gDNA was likely degraded. If you see no smears and no bands, then you may want to trouble-shooting using DNA samples with similar nanodrop reads and clear bands from your lab mates.

  Hi Zheng,

Firstly, thank you for your answer.

It is strange really I haven't had like that before at all. No Band and no smear. As you suggested, gDNA is degraded. But, how does it degrade while I did extract it freshly.

Other mates, extract DNA by CTAB using same solutions but for another fungi. I'm working with Fusarium.

Did you check your sample right after you made Nanodrop measurements? where did you keep your DNA sample? -20C?

When I did check my samples were in ice the I moved them to -20C.

dear sir firstly,

Thanks for all who tried to help me figure out this case.