I have PEGDA (mol wt - 6000) and Irgacure 2959 photoinitiator, could anyone tell me clearly how to make their solution and use them with collagen gel?
dear Tarun,
Irgacure 2959 photoinitiator is very low water soluble (1g/100 ml) but highly soluble in methanol and ethanol.
so dissolve both PEGDA and initiator in methanol or water/methanol combination system.
it will surely solver the purpose.
Wishes
Dr Maneesh Jaiswal
Biomedical Engineering, IIT Delhi
Turan,
Irgacur can be easily dissolved in DMSO at the concentration of 0,5 % w/v.
PEDdA can be dissolved in water. Pay attention to the concentration of PEGdA in solution. The mechanical strength of the hydrogel depends on the cross-linking density so, if you want to obtain soft hydogel, I recommend to not to exceed the concentration of 15% w/v of PEGdA in water. Ethanol is not recommended as it acts as a radical scavanger some times. Methanol is toxic for cells, so DMSO shall be the best choice if you are looking to prepare hydrogels for cell culture.
I hope that my answer could be useful.
Bests,
Irini Gerges, Ph.D.
You can solve the photoinitiator in water as far as it doesn't exceed 1%w/w and up to 90% of PEGDA in water.
Daniel Cárdenas, PhD(c)
North Carolina State University
Hello Tarun,
I would like to suggest not to use Irgacure due to its low solubility in water. better instead use LAP (0.25% wrt PEGDA). It has good water solubility as well in PBS.
Best wishes!
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