Could anyone suggest me an ideal protocol for sperm dna fragmentation in rats?

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Henry Clemente

We offer nanoparticles to deplete DNA damaged sperm

Henry [email protected]

Alexandre R. Garcia

I have used acridine orange protocols succesfully, but in bovine sperm.The protocol I have used in based on paper of UNANIAN (2000).

Eduard Sanchez

Hello Jimmy,

We sell a kit (GoldCyto-DNA) that works also in rat sperm. This kit is based in Sperm Chromatin dispersion test (SCD). You can see the protocol in this link:

http://www.micropticsl.com/wp-content/uploads/2013/09/goldcyto_dna_10_protocol.pdf

Our technology simultaneously targets 3 to 4 surface markers on sperm with our Nano magnetic particles. These targets are apoptotic sperm, acrosome reacted sperm and membrane damaged sperm. After a 20 minute incubation at room temp the mixture of particles and sperm are placed against a laboratory magnet for removal of the targeted deficient sperm. Essentially, the supernatant contains the viable sperm In so doing we enrich for viable non DNA damaged sperm as well as MMP sperm for use in IVF.