The number of bases is low, so I am unable to design a primer.
Please may I ask if non-salaried Public Contributors conduct data collection interviews or Focus Groups with NHS patients as part of your study? This means that the Public Contributor will either...
29 July 2024 3,085 0 View
Hi everyone, I am currently working on RNA extraction on tissue, but unfortunately, I only have large frozen clinical tissue on hand. In order to maximize the RNA integrity, I plan to use...
25 June 2024 7,550 1 View
i want to write literature review on the topic " production of 3d PLA filament"
19 June 2024 1,712 2 View
Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...
18 June 2024 6,854 3 View
In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...
15 June 2024 3,884 1 View
I have a set-up were I have a bottle with water (DI water, 0.8uS/cm). To that bottle I have added some MgO nanopowder, at a concentration of ~ 0.08mg/ml. It appears that the white powder is...
04 June 2024 9,789 4 View
As urbanization accelerates, the environmental toll of rapid urban growth becomes increasingly apparent. Please pitch your opinion/idea on this question as we explore innovative approaches...
02 June 2024 737 3 View
I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...
02 June 2024 5,373 2 View
My profile on researchGate is not yet visible on goggle
26 May 2024 5,824 1 View
Climate change-induced shifts in precipitation patterns are reshaping the availability and quality of freshwater resources worldwide. Join us in examining the far-reaching implications of these...
19 May 2024 8,764 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
