I'd like to isolate Actinomycetes isolates from the contaminated samples containing the gram negative bacteria and I tried different methods to get red of these gram negative bacteria and failed many times, so I want to use the most active antibiotics as Colistin and Polymyxin B to do so.
Could you help me with their optimum concentration which wouldn't harm my Actinomycetes isolates.
Thanks in advance
Dear Mohamed
I have used streptomycin (at final concentration of 50 micro gram/ml) and it worked for me. Don't autoclave antibiotic solution but filter sterilize it. if 50 mic didn't work then increase the final concentration up to 100 mic g/ml.
Hope this helps
Dear Mohamed,
for inhibiting growth of Gram negative bacteria in culture media, the most used antibiotics are colistin and polymyxin B. Using one of them should be enough. An amount of polymyxin B that usually works as gram negative growth inhibitor in culture media is 200,000 unit/L, (some 25 mg/L). This is, i.e., the concentration used in selective culture media for streptococci.
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