20 Questions 14 Answers 0 Followers
Questions related from Mohamed Sebak
How to merge the HRMS data in CSV files generated by MZmine (data processing) of both negative and positive modes in one CSV file containing the combined data in one datasheet to be used in the...
06 May 2020 4,694 0 View
I have done 16S rRNA sequencing for two actinobacteria isolates and when I tried the NCBI blast tool, I got hits for about 10 different species which are 100% identity, 100% query recovery and...
26 July 2018 6,120 3 View
I'm asking about the optimum oxygenation level or range for bioreactor fermentation of some soil actinobacteria for production of natural products. Thanks in advance
19 March 2018 5,137 7 View
I’d like to know how to prepare the standards strains broth in the best way and how to do the test and interpreter the results I know that it should be done in triplicates but I’m not sure about...
25 September 2017 639 11 View
I'd like to isolate Actinomycetes isolates from the contaminated samples containing the gram negative bacteria and I tried different methods to get red of these gram negative bacteria and failed...
29 July 2016 6,685 2 View
I have faced a great problem that my Actinomycetes isolates get contamination by gram negative bacteria many times. To face this problem I made isolation of them many times on ISP 4 agar...
09 February 2016 552 7 View
I have one Actinomycetes isolate and I tried to extract its DNA by heating method at 95 degree centigrade and also I tried this by Genomic DNA extraction kit of Sigma Aldrich. I used lysozyme...
29 December 2015 4,868 7 View
I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria What is the best way to do that and if I want to add antifungal in the media, what is the...
19 December 2014 8,122 11 View
If I want to extract large plasmids from bacteria (more than 100 kb), what is the best way and best kit to get them in pure and high yield. Please help me urgently if you can Best Regards
11 November 2014 8,467 15 View
I had isolated some isolates suspected to be actinomycetes. What is the best method for identification of them biochemically? Please help if you can Best regards
30 October 2014 7,722 2 View
If I want to obtain isolates of actinomycetes which produce novel biologically active secondary metabolites, what is the best media for cultivation of these actinomycetes to help them produce...
17 October 2014 2,011 16 View
If I want to have bacterial samples which can produce biologically active secondary metabolites, what is the best way to do that and what are the sources from which I can collect them? Please help...
15 October 2014 1,273 15 View
I have some gram negative isolates that I had tested for Antimicrobial activity production and a lot of them were having antimicrobial activity but after subculture of these isolates, many of them...
25 June 2014 258 5 View
If I want to extract the genomic DNA from bacteria, is the use of heating or boiling of bacteria an efficient method or should I try another method? The aim of extraction is PCR and sequencing/
15 April 2014 2,104 14 View
We have a big problem in our lab. After detection of the PCR product by agarose gel electrophoresis which contained the carcinogenic Ethidium Bromide, we face the problem of getting rid of it. We...
04 April 2014 2,407 5 View
When I was performing a PCR for some genes and running the PCR products on the agarose gel, I observed the presence of tailing and the bands were strong. Do you think I needed to change my PCR...
19 March 2014 1,591 10 View
If I want to isolate microorganisms producing antimicrobial natural products, what is the best source for these microorganisms I should isolate from; clinical, environmental or both? Please...
14 March 2014 4,326 5 View
Following a PCR, there were DNA bands on the agarose gel, but they were different than my specific band, as they appeared far from my target band.
26 February 2014 1,027 19 View
Does anyone know about the relationship between pigment production and antimicrobial resistance of Pseudomonas aeruginosa?
21 February 2014 6,412 2 View
After detection of biologically active peptides with antimicrobial activity, we need to fully identify them and know if they are new.
17 February 2014 4,428 3 View
