Should I use higher centrifuge speed or my polymerization actually did not work?

One of my sample (NIPs-non-imprinted polymer) couldn't settling down after centrifugation (4000 rpm). Might be due to very fine particles. So what I did, I let the samples to evaporate under fume...

06 December 2020 3,344 3 View

When I need to conduct a linearity test (e.g. Shapiro-Wilk test)?

Should I conduct a normality test (e.g. Shapiro-Wilk test) before conducting every parametric test (e.g. independent T-test, ANOVA or Pearson correlation) despite I have a large sample size (more...

22 October 2020 290 13 View

What can explain the high desorption percentage when the desorbent is NaOH on a material that has adsorbed an anionic dye ?

Good morning to all of you Please, I need some help, After the adsorption of an anionic dye by an activated carbon at acidic pH, I studied the desorption phenomenon using NaOH, H2O and H2SO4 as...

18 April 2020 2,592 5 View

How does the current flow in the tunnel junction of dual-junction solar cells?

The dual-junction solar cell composed of two sub-cells would achieve a better conversion efficiencies, but how does the current flow in the tunnel junction P++/n++ withou current loss?

12 April 2020 3,573 6 View

What's the best way to connect two solar cells in series?

We can connect CIGS/Pyrovskyte solar cells with several ways to form tandem solar cell. But what's the best one of them? . thank you.

08 April 2020 7,662 3 View

What's the best way to reduce the carrier recombination at the back contact of solar cells?

P-type absorber material, an n-type material is mandatory for creating a good p-n junction. The heterojunction Absorber/Buffer cell requires a transparent conducting oxide (TCO) layer at the front...

05 April 2020 7,047 4 View

How can help me about the real parameters for Perovskite thin film?

I would like to simulate a typical perovskite solar cell. I have found a lot of references for perovskite parameter values in literature. Does anyone have any reference so that I can get values...

02 April 2020 1,777 6 View

Can you tell me about the characterization methods to determine defects levels in the gap energy?

It's very important to determine the defects levels that impact on the solar cell performances, especially in the absorber part. We would like to know also the origin of this defects and their...

21 March 2020 4,938 7 View

How can I model & simulate quantum dote thin films using CASTEP with different tail parameters?

A QDSC solar cell design that uses quantum dots as the absorbing photovoltaic material. It attempts to replace bulk materials such as Si, CIGS, CdTe ... etc. Quantum dots have bandgaps that are...

16 March 2020 1,986 4 View

How I can simulate Quantum-well solar cells QWSC using silvaco?

I want to calculate the QWSC performance. The problem encountered is how to introduce the Quantum well in the structure . Can anyone suggest to me how can I simulate QWSC without any problem?...

07 March 2020 8,427 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View