There might be problems with many issues

1. When you run on gel do u get dimers as of high intensity or low. (If high it could be that the DNA might not be denatured so increase the temperature.

2. If you don't see any dimers at all then probably your primer is not annealing .....

Thanks a lot for your speedy reply Arun. :)

With those melting temperatures, you should definitely see something at 55C. THings you can try, off the top of my head:

Good luck

You can start first doing PCR with varying concentration of your DNA template with the normal annealing temp of your enzyme which I am guessing is 55C, and then if you find any PCR band with a specific DNA template concentration then you can play with the annealing temperature which will result with varying PCR band intensity. I normally do my PCR optimization or you can do both parameters at the same time. Wish you luck!

 Thank you Andrea Galli for your suggestions :). I am following the same procedure as you have suggested except i was using high DNA amount. I will try with less concentration of DNA in my next PCR experiment.

Hello Christian Cantos , your idea of using different concentrations of DNA is pretty exciting but i need to first optimize temperature in order to make my PCR work.. But Thanks a lot for replying :)

Hello Arthur Burzynski, I know melting temperature of our primers are quite high because we are also cloning ribosomal binding sites in our shuttle vector along with our gene of interest. It was really difficult for us to reduce the melting temperature while designing. Additionally, GC content is also pretty high in both of primers.I am really grateful for your suggestions Sir. Thank you again.