Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

How can I transfer heat between groups in Gromacs?

Is it possible to transfer heat from one group to another using simulated aneeling technique in the Gromacs software? I would like to transfer heat from a gold nanoparticle (group 1 from 310 to...

20 November 2020 9,062 3 View

What type of specimens do I need to take similar SEM pictures of petrified wood?

What would you advice me, what type of specimens do I need to take SEM pictures of petrified wood. do I need the polished samples, rough specimen or thin section?

30 November 2019 9,676 3 View

What are some economic incentives that make sustainable mining processes economically valuable for companies and communities?

Mining plays a significant role in regional development. It also generates great amounts of waste -- some of which contains toxic chemicals and heavy elements. Yet, additional mining-related...

11 August 2019 7,179 4 View

Does noise affect, or compound, mercury poisoning in the human body relative to small-scale gold mining?

On-the-ground experience would suggest that sound/noise pollution and mercury contamination may be coincidental in artisanal and small-scale gold-mining stakeholders and their bodies. If so, does...

31 July 2019 5,350 1 View

How can isolate red blood cells from non-heparinized blood stored in liquid nitrogen?

I am working with a fish and I want to measure the amount of DNA in the red blood cells using flow cytometry, to estimate the genome size. However, the blood from which i am trying to isolate the...

01 February 2019 3,548 3 View

How to integrate a function considering time and space (3D) in Comsol Multiphysics?

I'm working on a model in Comsol and one of my variables is the sample temperature. I need to integrate a function of temperature along the time and 3D-space. I've already tried the functions...

06 December 2017 5,384 2 View

How can I measure proliferation in co-culture without detaching cells?

16 February 2017 682 3 View

Who works with antimicrobial peptides used as antibiotics to treat lung infections?

Trying to make connections!!!

26 January 2017 5,283 4 View

Possible reasons for odd OD results in 24-well microplate?

I have recently started culturing different phytoplankton species in 24-well microplates and taking absorbance measurements using the microplate reader FLUOstar OMEGA. Surprisingly, OD data for...

13 June 2016 9,214 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View