Could anybody please send me the following two papers.

Sebastian Vencken @Sebastian_Vencken

08 August 2011 1 5K Report

I'd really appreciate it.

http://www.ncbi.nlm.nih.gov/pubmed/2438234

http://www.ncbi.nlm.nih.gov/pubmed/3204101

Many thanks

Badges
Science topic

Similar topics
Cell Biology
Methods

More Sebastian Vencken's questions See All

How can I shear RNA from formaldehyde-fixed cell lysates with Covaris M220?

I'm having some trouble obtaining an RNA 200-500 base fragment size in lysates from cells fixed with 1% formaldehyde. I've based the parameteres of my protocol on the Covaris protocol for Total...

10 November 2015 8,328 1 View

What is the stability of PLGA in chloroform?

I was hoping to prepare a long term stock of PLGA in chloroform, which I can store at -20C. Would anybody know what the long term stability of PLGA in chloroform would be? Thanks

06 July 2015 6,842 4 View

Secondary size peak for PLGA nanoparticles. Why?

I'm producing ~200 nm siRNA PLGA/DOTAP nanoparticles using the double-emulsion solvent evaporation technique. When I sized the particles using DLS the majority (95%) were of the correct size...

05 June 2015 7,195 9 View

How can we incorporate uncertainty in repeat experiments?

The authors of the following paper make some excellent points about the use of and difference between repeats and replicates: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321166/ They are very...

03 April 2014 2,346 13 View

Anyone have experience with trizol (phenol:chloroform) extraction while excluding small RNAs?

I'm aiming to attempt the opposite of what many on this forum are trying. I'm seeking to perform Trizol extraction with the implicit aim to purify RNA species that are longer than small RNA...

01 February 2014 9,203 3 View

Are there any reagents that can produce inter-RNA crosslinks that are reversible without having to introduce special nucleotides to the RNA?

Seeking information on Reversible RNA-RNA crosslinking

06 July 2013 7,100 2 View

Binding strength antibody vs oligonucleotide?

Would anybody perhaps know how an antibody compares to a short (~25 nt) oligonucleotide in terms of binding strength. I'm aware that this depends on many variables, but I'm just trying to get a...

06 July 2013 6,974 8 View

Psoralens and reverse transcription?

I intend to cross-link RNA using psoralens such as HMT. I would like to reverse these cross links, which I believe can be done with 254 nm UV or a base-catalysed reaction. I would like to create...

06 July 2013 7,942 0 View

Biotinylated oligonucleotide probe degradation?

I'm performing a RNA-precipitation protocol with specific biotin-TEG labelled probes which bind streptavidin magnetic beads. Over time my enrichments have become significantly lower. My suspicion...

05 June 2013 9,181 1 View

Aptamers or oligonucleotides?

A number of groups use aptamer-conjugated RNA sequences to specifically isolate RNA binding proteins. What is the advantage of using aptamers over e.g. using oligonucleotides to pull-down those...

04 May 2013 6,143 5 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View