Could any of you please share a protocol to make Mtb lysates and BCG lysates?

More Gishnu Harikumar Parvathy's questions See All

Are there lymph node mapping dyes that do not interfere with ELISA and Flow-cytometry based Multiplex assay?

I am trying to find axillary and mandibular lymph nodes in naive mice, which is turning out to be more difficult than imagined. Therefore, I would like to know if there are some possibilities of...

27 January 2021 9,669 3 View

How to find the mutual coupling effects of antenna arrays in CST Microwave Studio?

How can we calculate the mutual coupling effects of antenna arrays with single port in CST? The array consists of 4 element slots which is fed simultaneously using a corporate feed. What all...

21 December 2019 3,414 3 View

Metallographic preparation of Ti-Mo alloy?

I am currently working in Ti-Mo alloys with 25wt% and 30wt% Mo. Can anyone suggest a better method for the metallographic preparation of this alloy and a suitable etchant.

29 October 2019 7,786 8 View

How to constitute biological replications in RT-qPCR ?

In RT-qPCR can specimens/ samples from different plants be pooled to form a biological replication? If samples can be pooled from different individuals/plants, can the biological replications be...

01 January 1970 6,624 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Ofir Zavdy

Hope this helps:

Pratt Kolia-Diafouka et al. 2018:

"Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens"

Front Microbiol. 2018; 9: 2224

Gishnu Harikumar Parvathy

Thank you so much. It is indeed very helpful. Thanks. @Ofir Zavdy