Upon checking individual ingredient toxicity for baby oil/body wash, found to be nontoxic. Later after formulation, the finished product was found to be toxic. We performed MTT assay to check cytotoxicity using L929 cells.Can some help in this regard.
it depends on the type of material that be used in the formulation and product manufacturing step, it is necessary to study the interactions and degradation pathways of each material.
Hi Muthuraman,
A question, in order to search possible interactions, which are the ingredients involved?
Hello Muthuraman M
There is always a possibility that the interactions between different chemical components in a mixture may result in either a weaker (antagonistic) or a stronger (synergistic, potentiated) combined effect than the additive effect that would be expected from knowledge about the toxicity and mode of action of each individual compound. It is possible that individual ingredient may not be toxic, but the toxicity of a chemical could be enhanced when given together with another chemical.
These interactions may take place either in the toxicokinetic phase (i.e. processes of uptake, distribution, metabolism and excretion) or in the toxicodynamic phase (i.e. effects of chemicals on the receptor, or cellular target).
You need to study the interactions of various ingredients present in the product.
Best.
You may check all extraction procedures as example u can go through all the process with MTT assay at the se point u may reach the possible compound caused it to be cytotoxic with extraction
MTT assay using L929 cells@10 K is used., allowed to attach for 24 hrs. Sample preparation. 0.1 % weighed or pipetted and dissolved in DMEM medium with FBS and sonicated for 20 hz for 5 min. Then 100 ul loaded and treated for 24 hrs, then media is removed, washed twice using PBS. MTT reagent was added and then incubated for 3 hrs and so on.
Is this procedure is correct, mainly sample preparation?
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