Where to find photo dataset of depressed people faces?

Hi, for a facial emotion recognition detection project, a friend asked me if I know where he could find a dataset with faces of depressed people. I found this deep learning facial emotion...

02 June 2020 1,623 1 View

Predict solubility of organic compounds?

Is there a formula, or a program or web server, that will predict the solubility of an arbitrary organic compound in various solvents?

29 August 2018 6,298 3 View

Problem with cell death during long term imaging?

We have been using the cytation to image A375 cells over several days. Although it is CO2 and temperature controlled, we have been experiencing cell death. We have began adding HBSS to surrounding...

06 July 2018 667 3 View

How Long Does it take cells to recycle antibodies/ cell surface markers?

If antibodies are used for cell sorting, how long do they remain on the cell? How long does it take for these surface markers to be turned over? I am unsure if once cells are sorted if one can...

03 April 2018 6,425 2 View

Why lac repressor is being over expressed rather than my protein?

I've been trying to express eukaryotic phosphatase in E.coli bl21 de3 Plyss. I had initial success (30 mg) but now it seems that all I get is lac repressor when purifying the next batches. Growth...

09 March 2017 1,894 2 View

Any software recommendations for calculating various tests of selection?

I am trying to calculate various metrics related to population genetics using SNP datasets and full sequence alignments. I am looking for a program that can be run from the command line that can...

15 September 2016 5,588 2 View

What is the MMPI's measure aggression known as 'Pancheri's Index'?

29 May 2015 1,809 4 View

What is the MMPI's measure aggression known as 'Pancheri's Index'?

04 May 2015 419 5 View

Can anyone recommend an antibody for TMEM97 (transmembrane protein 97 also known as MAC30) for use in WB and ICC in fibroblasts and HeLa cells?

I am trying to do western blots and ICC for TMEM97 in different cell lines including human fibroblasts. Can anyone recommend one of the commercially available antibodies for this protein?

18 February 2014 7,384 3 View

Regarding nanocrystalline diamond vs diamond-like carbon, can someone help me about their deposition and properties?

To my knowledge, both materials can be deposited by plasma assisted CVD from generally the same precursors. Only the deposition conditions (like the way of plasma excitation and frequency and gas...

23 June 2013 5,025 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View