Does Anyone Know of a Way to Detect Melatonin that is NOT HPLC, RIA or Mass Spec?

I tried to do a Slot Blot and was not successful and melatonin is only .23 Kda so it would probably run off a western blot. Has anyone had success with other methods?

23 January 2021 1,388 3 View

Any advice or articles on combining one qualitative approach with the methods of a different approach?

I am a doctoral student and I am looking to do a qualitative research study that looks at the potential influence of teachers on the way minority students view their leadership capabilities....

28 November 2020 6,217 5 View

Troubles cloning - correct RE digestion and then incorrect sequencing?

I am inserting an MPP5 gene insert into a pCAGGs backbone. When I am finished with the mini prep, I find that my RE digestion is correct, but then when I send it to be sequenced, the MPP5 gene is...

23 November 2020 7,962 2 View

How to convert a cif file to a qe file?

I am trying to convert a cif file to a Quantum espresso file to do DFT calculations

10 September 2020 1,535 4 View

What are the available Nanocomposite Databases?

I am looking for data files of Nano Composites. Any form of data, be it XRD outputs, SEM or TEM images or anything. Where can I find them?

28 August 2020 9,927 5 View

Can I degas drug solutions before applying to mammalian cells for electrophysiology?

I am applying different drug solutions to cells that I am whole-cell patching. Each solution is in a syringe connected to a thin tube that goes through a barrel manifold into a glass micropipette...

24 August 2020 1,956 2 View

How to count % GFP positive cells and GFP intensity using imagej?

I have infected BHK21 cells with a virus that expresses GFP. I want to count the percentage of GFP positive cells and the intensity of the GFP. How do I do this with fluorescent microscope...

17 August 2020 4,009 2 View

CD8+ T cell and natural killer isolation kits from rat spleen?

Hello, Does anyone have any experience with isolation of CD8+ cytotoxic T cells and natural killer cells from rat spleen? I'd prefer kit-based if possible, but any protocols you can provide would...

30 July 2020 5,387 6 View

Best ways to analyze crowdsourced data for a qualitative study?

Hello, I am currently working on a project analyzing crowdsourced data from online trip reports and social media applications. However, I am struggling in defining the methodology for this...

25 February 2020 4,401 3 View

Porcine specific antibody that doesn't react with rabbit?

Does anyone know of an antibody that can be used for immunohistochemistry on paraffin embedded sections that reacts with porcine cells but not rabbit cells? We need to prove that the porcine cells...

20 January 2020 9,014 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

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17 February 2021 4,217 3 View