Contribution of serum lipid in Parkinson,s disease and other neuro degenerative diseases?

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Asking for a vector, containing Hygromycin B resistance gene?

In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...

19 June 2024 7,822 3 View

How can I plan for a PhD in tobacco related research in foreign universities?

Dear colleges, I'm a dental graduate (BDS) and did my master's degree in public health (MPH) with two years of experience. I'm working as a Scientist-B in one of the Indian Council of Medical...

02 July 2023 1,959 0 View

How to conduct Multiple correspondence analysis (MCA)?

Hi, To understand participants' life satisfaction, 5 questions have been asked. In each question, there are 5 options, from disagree to agree. Please suggest me how to create a single variable...

01 April 2022 7,014 5 View

Is there any relationship between land surface temparature and soil erosion? What do you think?

Recently, I have studied the impact of land surface temperature (LST) on an important soil erosion factor the cover management factor (C factor). My study was on the winter season. It shows a...

15 January 2022 4,397 3 View

Why in assembly cloning vector:insert is 1:4?

During setting up assembly cloning reaction, we need to add the insert and vector in ratio 1:4, which is mentioned in the protocol. But I don't know what is the reasons behind this ratio. I will...

28 June 2021 419 2 View

For a PhD scholar, what validity and reliability test should be good enough to call my tool a validated one?

For my Doctoral thesis, I am framing a quantitative questionnaire tool to collect healthcare seeking behavior of vulnerable tribal peoples. The content of questionnaire were synthesized from...

23 June 2021 8,853 4 View

Choice among r2 and RMSE for a good model?

I have generated so many models having a different coefficient of determination (r2) and RMSE. In this analysis, it has been seen that it is not mandatory that a model having maximum r2 has a...

15 July 2020 6,239 4 View

Why we should ignore multicollinearity?

In many situations in practice, the explanatory variables (xi) may not remain independent due to various reasons. The situation where the explanatory variables are highly intercorrelated is...

07 July 2020 6,689 21 View

How to convert gamma naught to sigma naught after RTC?

I am using ALOS-2 data. After radiometric terrain correction, I have got Gamma naught image. After that, I just want to have the Sigma naught image from that Gamma naught image.

03 May 2020 8,617 0 View

Is there any freely available InSAR processing software (Except SNAP, as it is highly RAM consuming and getting Java heap space error)?

I have stuck with the interferometry of Sentinel-1 data in the phase of coregistration. The SNAP gives java heap space error several times for two weeks. I am finding any freely available software...

20 April 2020 1,295 5 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

How can reinforcement learning algorithms be effectively applied to study decision-making processes in neuroscience?

I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...

17 July 2024 1,225 0 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View