10 March 2016 3 7K Report

Hi All, my dissertation is about filicide... im researching the factors that are behind/contribute towards this from a biopsychosocial perspective... thankyou

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What is the cause of streaking and double banding on gels?

I'm working on library prep for ITS NGS using Earth Microbiome Protocol and am getting double banding and smearing on my gels. What might be the cause for this? I should be seeing a band around...

04 March 2024 5,940 1 View

How long do unused tissue slides for IHC last?

Hi All, I am wondering how long are unused tissue slides, used for IHC, last at 4 degrees? I see conflicting reports in the literature. I have 1 yr. old tissue slides stores at 4 degrees. Am I...

19 July 2021 5,956 5 View

Dapi or Hoechst in live cell imaging?

Hi there. I am performing some life imaging experiments and would like to look at the nucleus using either Dapi or Hoechst. I am wondering if anyone could share a protocol. My understanding is...

07 June 2021 6,261 3 View

Human CAR in mouse T cells?

Is it possible to transduce a human CAR into mouse T cells and have the mouse T cells function when the CAR binds it's antigen? I am thinking that if anything the singling domain of the CAR, as it...

10 May 2021 6,131 2 View

How can you recover BPB after release of amino acid from Ni Schiff base complexation?

Many papers say they recovered the initial BPB (S)-2-[N-(N′-benzylprolyl)amino]benzophenone ligand after getting their amino acid from the Ni (II) Schiff base complexation, but I can't find...

19 April 2021 3,297 1 View

Particle Size Analysis,DLS, of Hetergeneous Oxidized Carbon Based Particles; what factors could produce ope-ended particle distribution curves?

Hello, I am somewhat new to dynamic light scattering. I am working with a heterogeneous mixture of carbon based particles synthesized at various hydrogen peroxide concentrations. It's worth noting...

12 July 2020 905 2 View

Recommended cutpoints for hip-worn actigraph measuring physical activity in pregnant teens?

From the literature we've seen research mostly examines pregnancy in adults (>20 years), but our population is 14-18 years old. We'd love any feedback on if we should go with cutpoints for...

09 June 2020 3,064 1 View

What bacteria could this be?

I'm trying to identify the species of even genus of this bacteria I have grown in the lab but due to its unique colony appearance the bacteria I am getting from decision trees are not showing any...

25 November 2018 5,040 0 View

How to serum starve the cell lines bEnd.3 and sEnd.1 (middle t antigen expressing)?

I've been working with bEnd.3 and sEnd.1 cells which are endothelial cell lines from the periphery (sEnd1.s) and the brain (bEnd.1s). They are immortalised using middle T antigen. I need to serum...

22 March 2018 9,297 3 View

Quantification of rat corticospinal neurons?

Hello, Does anyone know of any studies that counted corticospinal axons in cross section throughout the levels of the spinal cord? Thanks, Bethany

08 February 2018 7,408 3 View

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