Validation of tensile experimental result with abaqus?

Hello, I have stress-strain graph extracted by the tensile test. I also model the same with abaqus. I want to get the stress-strain graph using the abaqus. Does anybody know how can I do that?...

31 December 2019 1,613 4 View

Choose shell part in abaqus while assigning solid homogeneous section?

Hi, I have a question regarding section assignment in abaqus. Does anybody know why we should assign solid homogeneous section for a part which is designed as 2-D planar deformable shell? Thanks

31 December 2019 3,654 4 View

3D composite analysis abaqus?

Hello, Does 3D composite analysis in Abaqus, behave exactly like composite materials? I saw somewhere that they use solid homogeneous and then partitioned the part and then assign material...

11 December 2019 9,184 2 View

Analyzing problem while Importing geometries and models in Abaqus from Texgen?

Hello, Does anybody have experience to import weaven textile model from texgen to abaqus? I imported it. It is imported as orphan mesh that you cannot edit the geometry. I dealt with that and...

11 December 2019 7,245 3 View

Extrapolate stress values from center of the elements to the nodes?

Hi, Does anybody have any idea how can I extrapolate stress values from center of each elements to the nodes? It seems that it is required to have a contour plot of stress for those elements. Thanks

11 December 2018 7,492 0 View

Assigning boundary conditions for a plate element?

I have a problem assigning boundary conditions in different nodes in plate element. When we say that two edges are simply supported and two others are clamped, how should we assign boundary...

07 August 2018 9,433 1 View

Stiffness assembly for the BCIZ (triangular element) in plate bending problems?

Hello all, Does anybody have any information about MATLAB coding for assembling the stiffness matrix of the triangular element for plate bending problem? (9 DOF element). Thanks

04 May 2018 5,542 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View