How to separate protein-RNA complex?

I am purifying protein which has RNA in its bound form. How can I remove this bound RNA from protein without affecting structure of protein. RNase treatment is not much effective.

31 December 2018 7,802 4 View

Protein complex in Native PAGE?

I am trying to separate protein complex and protein monomer in native PAGE but protein is not able to move inside the gel and it get stuck at well only. I tried with 5% acrylamide, 5% glycerol,...

04 May 2018 5,720 5 View

Any expereince about gluteraldehyde crosslinking?

I am trying to crosslink my protein by gluteraldehyde but after crosslinking multimeric form of protein is not able to run on SDS PAGE even after 6 hrs long run at 90V. Please share your experience.

02 March 2018 2,032 4 View

PSUMO PRO vector cloning?

Hello, Does anyone have experience in ligation of >1.5kb gene in pSUMO PRO vector or any other vector. Please give suggestions.

10 November 2017 8,341 4 View

Increase RNA concentration?

Which method is more suitable for purification of RNA so that I can get a high concentration of RNA along with good volume. Please suggest.

08 September 2017 765 2 View

Ligation independent cloning ?

Can anyone provide basic experimental setup for ligation independent cloning method. I am not able to understand the basic mechanism behind this method.

08 September 2017 2,534 14 View

Restriction sites known but getting unknown gel band. How?

I digested my circular plasmid having 1 RE site for BamH1 and 1 for Bsa1. So I supposed to get 2 bands but getting one more low molecular weight band. Please explain any reason.

11 December 2016 3,227 16 View

Rosetta cells in autoinduction media?

Hello I have cultured rosetta cells in ZYP5052 autoinduction media. It is showing very slow growth. Does anyone have some experience with this media? please share .

11 December 2016 7,177 3 View

How addition of Arg + Glu increase protein solubility?

If Arg and Glu are added to purified protein it increases the solubility of it. Why?

08 September 2016 9,249 4 View

Solubility of Protein on lower temperature?

I have read in experimental procedure that lowering the temperature will translate more soluble protein. What is the reason behind it ?

07 August 2016 6,348 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View