Akshay Pareek

11 Questions 12 Answers 0 Followers

Questions related from Akshay Pareek

How to separate protein-RNA complex?

I am purifying protein which has RNA in its bound form. How can I remove this bound RNA from protein without affecting structure of protein. RNase treatment is not much effective.

01 January 2019 7,855 4 View

Protein complex in Native PAGE?

I am trying to separate protein complex and protein monomer in native PAGE but protein is not able to move inside the gel and it get stuck at well only. I tried with 5% acrylamide, 5% glycerol,...

05 May 2018 5,805 5 View

Any expereince about gluteraldehyde crosslinking?

I am trying to crosslink my protein by gluteraldehyde but after crosslinking multimeric form of protein is not able to run on SDS PAGE even after 6 hrs long run at 90V. Please share your experience.

03 March 2018 2,087 4 View

PSUMO PRO vector cloning?

Hello, Does anyone have experience in ligation of >1.5kb gene in pSUMO PRO vector or any other vector. Please give suggestions.

11 November 2017 8,395 4 View

Increase RNA concentration?

Which method is more suitable for purification of RNA so that I can get a high concentration of RNA along with good volume. Please suggest.

09 September 2017 825 2 View

Ligation independent cloning ?

Can anyone provide basic experimental setup for ligation independent cloning method. I am not able to understand the basic mechanism behind this method.

09 September 2017 2,593 14 View

Contamination in Rosetta?

I am inoculating Rosetta strain in LB broth but everytime culture got contaminated. Even after fumigation of lab. Please suggest any reason or solution.

04 April 2017 1,040 11 View

Restriction sites known but getting unknown gel band. How?

I digested my circular plasmid having 1 RE site for BamH1 and 1 for Bsa1. So I supposed to get 2 bands but getting one more low molecular weight band. Please explain any reason.

12 December 2016 3,288 16 View

Rosetta cells in autoinduction media?

Hello I have cultured rosetta cells in ZYP5052 autoinduction media. It is showing very slow growth. Does anyone have some experience with this media? please share .

12 December 2016 7,260 3 View

How addition of Arg + Glu increase protein solubility?

If Arg and Glu are added to purified protein it increases the solubility of it. Why?

09 September 2016 9,302 4 View

Solubility of Protein on lower temperature?

I have read in experimental procedure that lowering the temperature will translate more soluble protein. What is the reason behind it ?

08 August 2016 6,404 7 View