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What are the challenges or short falls with the ISO 45001?

In 2018 the OHSAS 18001 was improved and progressed into ISO 45001. This was characterized by one or two short falls. I am now reviewing the ISO 45001, with the aim of looking at the entire...

18 July 2024 9,489 0 View

What are the estimation methods and their properties, and the edge they have over others, especially the maximum likelihood estimator?

what are the estimation methods and their properties, and the edge they have over others, especially the maximum likelihood estimator? what is multinomial logistic regression and its properties...

03 May 2024 1,261 1 View

How can healthcare systems collaborate to enhance the comprehensiveness and utility of regional antibiograms in guiding antibiotic therapy?

This question prompts exploration into collaborative approaches to improve the quality and relevance of regional antibiograms. This encourages critical thinking about potential strategies for...

11 April 2024 2,770 0 View

Please, I need a suggestion on the most recent and best(suitable )PHREEQC database for surface-groundwater mixing/modeling?

The parameter of interest includes; Major ions and TROCs.

01 April 2024 8,466 1 View

Is there anything like "indigenous Multilingual Pronunciation"?

In a multilingual setting, Is there anything like "indigenous Multilingual Pronunciation"?

30 January 2024 8,287 1 View

You requested I verify I am elgible to post, but the link did not work. How do we fix this?

My name is Kenneth Neil Swartz, and I have numerous articles already posted on Research Gate wit a co-author. Below are some examples. Please approve my account so I can post a paper as a...

30 January 2024 9,593 0 View

Evaluation of an HSE management system ?

How is an HSE management system evaluated, to trigger a sustainable workplace?

24 January 2024 2,121 2 View

Why do I get more than 100% in my upper plateau when determining IC50?

I am working on an inhibition assay. I included 10-12 concentrations for the assay to account for the upper and lower plateaus of the sigmoid curve. To make the calculation of IC50 easier, I used...

27 August 2023 2,986 0 View

Is there a way to double the length of SD error bars in GraphPad Prism?

Some of my data points are outside the standard deviation, so I want the error bars to capture them by doubling the SD values. Is there a way to do this? How? If not in GraphPad, how about in Excel?

31 July 2023 4,755 2 View

Is there a reliable protocol for extraction of cell free mRNA from blood plasma stored at -80C in EDTA ?

Hello, I am planning an experiment. I may be able access blood plasma samples. These samples were collected from patients in EDTA tubes, centrifuged and the plasma supernatant extracted and...

26 June 2023 312 0 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Which software tools are best for enhancing diagnostic accuracy in chest X-ray imaging using image reconstruction and neural networks?

I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...

22 July 2024 3,794 1 View

Analysis of MHC-I and II alleles with CNVs and unassigned loci?

I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...

15 July 2024 182 1 View