Ibrahim thank you for your answer. Actually am using allelic SNP markers after from which it was sequenced. I finally overcome the problem by deleting the number of missing values (loci). After the data was cleaned, I was able to get the tree.
About the PCA, can it be calculated from the DARwin software as well?
Dear Caled, Could you please let me know how did you remove the missing SNPs from your sequence data! I have sequence of 98 samples and when I filtered them for less than 10% missing data no sample remains. What should I do?