Publishing in F1000Research??

Hi, Anyone has first hand experience of publishing in F1000Research. I guess it follows open peer review and does not encourage measurable parameters like impact factor! What are pros and cons...

05 June 2019 9,547 1 View

Protein-protein interaction??

Hi, I have two proteins that are his tagged at N-terminus. One of them forms hexamer around 370kDa and other is 17kDa. I want to check their interaction and get Kd value. Which of the following...

03 April 2019 1,474 5 View

Designing mutation experiments??

Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...

02 March 2019 4,847 5 View

E.Coli expression strain for expressing mycobacterial membrane protein gene?

Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...

11 December 2018 1,871 3 View

Bioinformatic tool to predict flexible region in a protein?

Hi, Can anyone please tell some bioinformatics server that helps in predicting the location of flexible region in a protein.?

10 November 2018 6,510 0 View

Difference between Coomassie Brilliant Blue R-250 and G-250??

Hi, Can anyone please highlight the difference between Coomassie Brilliant Blue R-250 and G-250?? Which one of them will stain the SDS gel faster?

09 October 2018 2,780 0 View

Citing preprint articles?

Hi, I was just curious to know whether the scientific articles and review published in preprint repository like bioRxiv are citable? If yes, is it good practice to do that since mostly such...

09 October 2018 4,686 4 View

How to degass a buffer with detergent?

Hi, I want to add about 5 CMC DDM detergent in my Size exclusion buffer. Is it advisable to add detergent and then degass by vacuum or degass the buffer first and then add DDM dropwise slowly??

08 September 2018 4,062 3 View

1M NaOH cleaning overnight?

Hi, One of my colleague put the Superdex 200 10/300 GL column mistakenly in 1M NaOH at flowrate of 0.2ml/min overnight instead of water wash. Will the column be stable? How to know that the...

07 August 2018 5,171 1 View

Publishing in bioRxiv??

Hi, Can someone please tell pros and cons briefly of publishing in preprint servers like bioRxiv?

07 August 2018 7,602 1 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

No title

03 March 2021 5,360 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View