03 March 2018 1 4K Report

Hi,

To concentrate a protein, what speed should be used in centrifugal filter? Does protein stick to membrane? Some people say that concentrating your protein will lead to aggregation, is it true??

More Haaris Ahsan Safdari's questions See All
Publishing in F1000Research??

Hi, Anyone has first hand experience of publishing in F1000Research. I guess it follows open peer review and does not encourage measurable parameters like impact factor! What are pros and cons...

05 June 2019 9,763 1 View

Protein-protein interaction??

Hi, I have two proteins that are his tagged at N-terminus. One of them forms hexamer around 370kDa and other is 17kDa. I want to check their interaction and get Kd value. Which of the following...

03 April 2019 1,604 5 View

Designing mutation experiments??

Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...

02 March 2019 4,959 5 View

E.Coli expression strain for expressing mycobacterial membrane protein gene?

Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...

11 December 2018 1,979 3 View

Bioinformatic tool to predict flexible region in a protein?

Hi, Can anyone please tell some bioinformatics server that helps in predicting the location of flexible region in a protein.?

10 November 2018 6,649 0 View

Difference between Coomassie Brilliant Blue R-250 and G-250??

Hi, Can anyone please highlight the difference between Coomassie Brilliant Blue R-250 and G-250?? Which one of them will stain the SDS gel faster?

09 October 2018 3,062 0 View

Citing preprint articles?

Hi, I was just curious to know whether the scientific articles and review published in preprint repository like bioRxiv are citable? If yes, is it good practice to do that since mostly such...

09 October 2018 4,812 4 View

How to degass a buffer with detergent?

Hi, I want to add about 5 CMC DDM detergent in my Size exclusion buffer. Is it advisable to add detergent and then degass by vacuum or degass the buffer first and then add DDM dropwise slowly??

08 September 2018 4,241 3 View

Publishing in bioRxiv??

Hi, Can someone please tell pros and cons briefly of publishing in preprint servers like bioRxiv?

07 August 2018 7,726 1 View

1M NaOH cleaning overnight?

Hi, One of my colleague put the Superdex 200 10/300 GL column mistakenly in 1M NaOH at flowrate of 0.2ml/min overnight instead of water wash. Will the column be stable? How to know that the...

07 August 2018 5,344 1 View

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