Why not you try this with batch mode ! ( HTPD : high throughput process development)

1 : Clarify your sample with centrifuge or filtration to remove suspended insoluble particles and impurities.

2. Find out approximate pI and molecular weight of your target enzyme by Electrophoresis or by literature.

3. If target compound pI is acidic try Anion Exchange ( strong Q+ and weak DEAE+) , If your target compound pI is basic try Cation Exchange ( Strong SO3- and weak CM-) Keep the pH and conductivity of loading buffer and your crude protein ( conductivity < 5 mS/cm)

4.Screen the proper pH and conductivity with consideration of stability of your protein and buffer pka.

5.there are so many Ion exchanger available in the market. You can screen Anion Exchange resin in batch mode as per availability :

(Ge) Q Sepharose™ FF , Q Sepharose™ HP, Q Sepharose™ XL, DEAE Sepharose™ FF ,ANX Sepharose™ 4FF low sub, ANX Sepharose™ 4FF high sub, SOURCE™ 15Q,,SOURCE™ 30Q, Capto™ Q , Capto™ DEAE, Capto™ Q ImpRes ,

(Merck Millipore) Fractogel® EMD DMAE (M), Fractogel® EMD DEAE (M), Fractogel® EMD DEAE (S), Fractogel® EMD TMAE (M), Fractogel® EMD TMAE (S)

Fractogel® EMD TMAE Hicap (M), Fractogel® EMD TMAE Medcap (M), Eshmuno™ Q

(Avantor)BAKERBOND™ POLYPEI ,

(Biorad)Macro-Prep® DEAE, Macro-Prep® High Q , Macro-Prep® 25 Q, UNOsphere™ Q, Nuvia™ Q,

(Chisso )Cellufine® MAX Q-r , Cellufine® MAX Q-h , Cellufine® Q-500 (m) ,

(Life T) POROS® 50 HQ, POROS® 50 PI, POROS® 50 D, POROS® 20 HQ

(Pall) Q HyperCel™, DEAE Ceramic HyperD® F , Q Ceramic HyperD® 20, Q Ceramic HyperD® F ,HyperCel™ STAR AX

(ProMetic) DEAE PuraBead HF

(Tosoh) Toyopearl® DEAE-650C , Toyopearl® DEAE-650M , Toyopearl® DEAE-650S , Toyopearl® SuperQ-650C , Toyopearl® SuperQ-650M , Toyopearl® SuperQ-650S , Toyopearl® QAE-550C , Toyopearl® GigaCap® Q-650M , Toyopearl® Q-600C AR

( YMC ) YMC - BioPro Q30 , YMC - BioPro Q75

3. Find out stability window of pH , conductivity , temp etc of your protein by analysis.

4.Your purity of target enzyme depend on Host related impurities, process related impurities , product specific impurities. Understand more about your target enzyme and impurities.

5. After confirming from batch mode , you can try bind and elute mode or flowthrough mode.

Go through with attachment for more information or write me more details of your target compound and impurities to [email protected]

Best luck.

Thank you very much for your kind answer, I will definitely refer to you if I have more questions