Can I compare flow samples run on different days if I use the same compensation matrix?
The samples are from the same experiment, I just didn't have enough cells for one sample to analyze it with the rest. Can I import the 1st day's experiment into FacsDIVA, use the same compensation matrix, and analyze one more sample on a different day?
It would be on the same machine, same voltage settings, same antibodies/dyes, same number of cells, and our flow core validates voltage consistency daily.
Saleh Alkarim
Hello
Yes i agree with Shiqiu Xiong , you can do that
look at book here may help
Good Luck
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Kevin Marron
You might consider preserving all samples in paraformaldehyde prior to running them? they will all be analysed under the same conditions whenever you get to run them
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