Kelly McGlynn

3 Questions 5 Answers 0 Followers

Questions related from Kelly McGlynn

Leave GFP uncompensated during flow cytometry?

I'm doing a differentiation experiment with flow analysis, comparing two cell lines: one is the wt cell line, and the other is a stable line transduced with our GOI + GFP marker. The GFP is just a...

25 August 2017 1,748 3 View

Compare flow samples run on different days?

Can I compare flow samples run on different days if I use the same compensation matrix? The samples are from the same experiment, I just didn't have enough cells for one sample to analyze it with...

26 May 2017 480 3 View

Stable cell line infected with gene-BFP, BFP visible but no protein on WB?

I infected MEL cells with lentivirus encoding my protein of interest (Flag tagged) with a BFP marker. So the construct goes EF1a promoter-gene-Flag, PGK-BFP-puro. I selected the cells with...

15 March 2017 8,133 2 View