J'ai lancé une qPCR sur mes échantillon d'Arabidopsis, avec différents génotypes, et j'aimerais savoir comment le logiciel de qPCR quantifie le nombre de transcrits dans mes différents échantillons.
Inspiration is the TLS Continuum Empowerment Model by Daniel T. Bloom
08 May 2022 3,482 4 View
Hey guys, atm I'm planning my tube formation with a new setup because I got positive results in my negative ctrl therefore I probably need to starve them to see the effect only in the cocultures....
06 April 2021 8,972 1 View
Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...
09 February 2021 7,176 3 View
Hello everyone, I'm looking for a 3D modeling software to do Zstack reconstructions (cells) . As a student I am looking for something that is open access and free. I know that imagej offer this...
24 March 2019 501 3 View
Hi Academic community! What are, according to you, the best sources on meta-analytic path models and full MA structural equation models? I am looking for both founding fathers of this methodology...
12 February 2019 1,034 2 View
Any research on modeling skew Gaussian spatial data in the presence of censored data?
15 December 2018 4,443 3 View
Any ideas on where I can register my meta-analysis? I am familiar with the PROSPERO and Cochrane initiatives but my meta-analysis is situated in political psychology (attitudes as dependent...
05 August 2018 6,801 3 View
When treating my cells I could observe a significantly reduced proliferation rate compared to the untreated control cells (could be confirmed by manual counting and MTT). When doing FACS analysis...
03 June 2018 1,683 5 View
I am performing a meta-analysis using the Comprehensive Meta-Analysis software. However, I am not sure how to enter DID estimates. Anyone here who knows which exact option it is in CMA's...
30 May 2018 4,557 7 View
Dear all, one of my customer had an original problem with his swimming pool since almost 2 years ! The swimming pool shown a strong contamination with bacteria, rotifers and helminth eggs....
19 April 2018 6,612 6 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
En suivant toutes les étapes mentionnées sur (ajouter une recherche) il me signale erreur, ressayer, j'ai essayé plusieurs fois, sans succès, toujours il me signal erreur, ressayer
02 August 2024 9,719 1 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
23 July 2024 9,421 3 View
La politique de financement est en soit une politique publique. Comment les évaluer dans un travail de recherche scientifique ?
22 July 2024 6,495 2 View
One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...
20 July 2024 3,392 2 View
Phase quantification in steel contain BCC and FCC
20 July 2024 8,150 7 View
It happened that I am asked to review a paper for a journal. Incidentally, the paper is posted as a preprint in another database as open access with DOI. It is completely citable. Now, should I...
17 July 2024 1,831 4 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View