Oh Mr. Mozhi, it dosent matter. In colorimetric method we can be analyze the ethanol concentration by using p- amino benzoic acid diazotized form (PABA) complex. It shows the accuracy in milimole concentration. Though, the standard preparation aqueous polar solvent might freely soluble of ethanol with a complex PABA show the absorbance at 468 nm scale, but dilution is show the good results (miliMol concen better yield in Beers law). If you feel PABA is costly simple iodometric titration can be use to identify the amount of ethanol by dichromate test. It is freely available in all chemistry lab and the procedure is very simple. Titrate the approximate quantities of ethanol and dichromate by 2:3 ratios. Liberate the amount of chromium ion titrate with iodide liberates iodine back titrate with sodium thiosulphate you get an equal to the amount of ethanol content.

@Hariharan,

Thanks for the reply,

I found this method while searching, can you suggest this method, it has a quantitative range between 0.0010 to 5.18 mg/ml.

Williams, M. B. and Reese, Darwin, Colorimetric determination of ethyl alcohol, Anal. Chem., 22, 1556, 1950.

I want to know whether international journals will accept this method in our research paper.

Yah mozhi, i feel it is very high concentration as you mentioned that range in ml, it not be accepted in lab scale results, use the dilution factor by analysis. Our true experimental results wont be conflict for acceptance by any journals, but they want accuracy arguments about them comments and expect some sophisticated quantification analysis reports. I helpless to suggest whether the journal accept or not, because of our good scientific paper was rejected some unreasonable reasons. I better suggest you, first focus the scope of your work on the appropriate journals, which will be helpful to you the way of easy publications.

Once again thanks a lot for your suggestions.

yah, its my pleasure Mr. Mozhi, you almost welcome. Take care.

Enzymatic method coupled to application of some redox dyes could be applied. Here is one example, in YOURS case YOU need to exchange the glucose oxidase by alcohol oxidase, which is also easily available in research labs and probably even commercially:

Article Spectrophotometric evaluation of gold nanoparticles as red-o...

I am currently using iododmetric method and it works. it is very accurate and simple.

Dear Eyiuche Julius Nweze,

Would you please give the protocol, I am also working with lignocellulosic materials to produce ethanol and for my screening I need to determine the alcohol amounts produced by my strains.

I also studied this article " Williams, M. B. and Reese, Darwin, Colorimetric determination of ethyl alcohol, Anal. Chem., 22, 1556, 1950. " but it is a bit complicated for me.

Dear Eyiuche Julius Nweze,

Would you please to share the protocol using iodometric method?, I need to cuantify ethanol from some fermentations with yeast and bacteria. Thank you very much.

Kind Regards.

Marco Fernández

A method for measuring the ethanol concentration in a yeast culture broth was developed using both microtubes and a 96-deepwell microplate. The strategy involved the solvent extraction of ethanol from culture broth and measurement the ethanol concentration by using the dichromate oxidation method. The article has been attached.