I am using DMH to induce colorectal cancer with 20mg/kg dose twice a week for two weeks subcutaneous injection..
Please, refer me how to be sure that my injection is right and the accurate subcutaneous injection, and give me any helpful advice it is my first research.
thanks a lot
DMH is the most widely used animal model for colon cancer and this chemical induces colon adenocarcinoma with highest incidence rate..
In general it is given as intraperitoneal injection for about 4 months followed by confirmation of colon cancer.
A work by my colleague (link below) may be helpful to you. Evaluation of crypt foci is a major and useful parameter as well.
https://www.researchgate.net/publication/274141850_In_vitro_and_in_vivo_evaluation_of_novel_cinnamyl_sulfonamide_hydroxamate_derivative_against_colon_adenocarcinoma
Best Regards,
Grandhi V Ramalingayya
Article In vitro and in vivo evaluation of novel cinnamyl sulfonamid...
Dear Seham
I am also working on colorectal cancer n i am using DMH for the (20mg/kg body wt) but my protocol is little diffrent from yours,I used give subcutaneous injection of DMH once in a weak till 16 week..to conform subcutaneous injection use small bore needle while giving injection maitain 15 degree angle. Thanking You..
Gud luck..
DMH is the most widely used animal model for colon cancer and this chemical induces colon adenocarcinoma with highest incidence rate..
In general it is given as intraperitoneal injection for about 4 months followed by confirmation of colon cancer.
A work by my colleague (link below) may be helpful to you. Evaluation of crypt foci is a major and useful parameter as well.
https://www.researchgate.net/publication/274141850_In_vitro_and_in_vivo_evaluation_of_novel_cinnamyl_sulfonamide_hydroxamate_derivative_against_colon_adenocarcinoma
Best Regards,
Grandhi V Ramalingayya
Article In vitro and in vivo evaluation of novel cinnamyl sulfonamid...
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